This book compiles research aspects of second-generation (2G) biofuel production derived specifically from lignocellulose biomass using biorefinery methods. It focuses on the valorization of different sources of 2G biofuels and their relative importance. The constituents of lignocelluloses and their potential characteristics different methods of treating lignocellulose, various means of lignocellulose bioconversion, and biofuel production strategies are discussed.Features:Describes technological advancements for bioethanol production from lignocellulosic wasteProvides the roadmap for the production and utilization of 2G biofuelsIntroduces the strategic role of metabolic engineering in the development of 2G biofuelsDiscusses technological advancements, life cycle assessment, and prospectsExplores the novel potential lignocellulosic biomass for 2G biofuelsThis book is aimed at researchers and professionals in renewable energy, biofuel, bioethanol, lignocellulose conversion, fermentation, and chemical engineering.