This volume details procedures relevant to all disciplines, with specific emphasis on challenging aspects of working with Bacterial Extracellular Vesicle (BEVs). Chapters are divided into four parts focusing on characterization of Bacterial Extracellular Vesicles, assessment of Bacterial Extracellular Vesicle Biological Function In Vitro, preparation and functionalization of therapeutic Bacterial Extracellular Vesicles, and delivery and tracking of Bacterial Extracellular Vesicles In Vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Extracellular Vesicles: Methods and Protocols aims to ensure successful results in the further study of this vital field.